OptiMax™ Automation ELISA Plate Adoption Kits provide all the materials and methods required to adapt your immunoassay from conventional 96-well or 384-well ELISA plate format to OptiMax™ microfluidic plate format in just three easy steps, requiring less than a day to perform. This kit enables optimization of the assay (including coating buffer selection, antibody cross-titration, and dynamic range definition) entirely using robotics and automated pipetting from start to finish; no manual assay work is required.
Set Up: Verify the pipetting operation of your instrument with OptiMax™ plates and run the control assay included in the kit provided to adjust flourescence plate reader settings.
Step 1: Select Optimal Capture Antibody Binding Buffer (OptiBind™ Buffer Screening)
A simple one-run protocol identifies the best OptiBind™ buffer to be used for each assay of interest.
Step 2: Select Optimal Antibody Concentrations (Antibody Cross-Titration)
An expedited protocol employing a DOE-based antibody concentration optimization matrix suitable for automation efficiently identifies optimal antibody conditions.
Step 3: Tune assay for desired Sensitivity, Sample Utilization and Throughput
Set desired standard curve range with tunable sensitivity, based on number of sample/standard reloading iterations. Limit of detection correlates directly with number of sample/standard reloads, hence sensitivity is “tunable”, a unique attribute of Optimiser™ Technology.
OptiMax™ Automation Plate Adoption Kit:
Components: 5 plates and sufficient buffer and substrate reagents to transfer assays of interest
- One plate for liquid handling, tip adjustment, maximum RFU sensitivity adjustment
- One plate for control sandwich immunoassay to adjust and verify flourescence plate reader settings (gain/sensitivity).
- One plate for binding buffer selection
- One plate for antibody titration and optimal concentration selection
- One plate to run optimized assay to determine dynamic range and LOD
|Catalog No.||Product Name||Price|
|OMP-AK||OptiMax™ Automation Plate Adoption Kit||$495.00||currently out of stock|