Assay SolutionsExperience the power of Optimiser™ Technololgy with our OptiMax™ Assay Kits and Optimiser™ technology- specific assay protocols. Siloam continues to developed Optimiser™ based protocols for a wide range of ELISA assays using commercially available antibody pairs.

Achieve Superior Performance, Save Sample, Increase Throughput and Get More Mileage out of your Reagents. Optimiser™ based protocols:

  • 5 μl sample volumes
  • 2 hours from assay start to assay results
  • 4 to 10x more tests with your current reagents
  • Expanded Dynamic Range
  • Enhance Sensitivity

Is your assay of interest not listed? Please click here to request the development of an Optimiser™ protocol for your specific research interest.

Click on application note code for details.
Application notes and performance claims are based upon Optimiser™ microplates used in manual mode. The following assays are easily transferable to OptiMax™ Automation Plates for even greater performance enhancement.

Application Note Species Analyte Vendor Vendor Catalog # Sensitivity (5 μl) Sensitivity (100 μl) Reagent Savings
AN0100 Human TIM-1 R&D
Systems
DY1750 31.2 pg/ml 1.6 pg/ml 75 assays for the cost of 15
AN0101 Mouse TGF-β1 R&D
Systems
DY1679 31.2 pg/ml 1.6 pg/ml 75 assays for the cost of 15
AN0102 Mouse IL-23 R&D
Systems
DY1887 39 pg/ml 1.95 pg/ml 150 assays for the cost of 15
AN0104 Human c-Met (Total) LIFE
Technologies
CHO0285 0.78 ng/ml 0.039 ng/ml 10 assays for the cost of 1
AN0105 Human IFN-α (subtype 2) Mabtech 3423-1H-6 1.56 pg/ml 0.08 pg/ml 10 assays for the cost of 1
AN0106 Human VEGF LIFE
Technologies
CHG0113 23.4 pg/ml 1.2 pg/ml 10 assays for the cost of 1
AN0107 Mouse IL-6 LIFE
Technologies
CMC0063 15.6 pg/ml 0.8 pg/ml 25 – 50 assays for the cost of 10
AN0108 Human Prolactin R&D
Systems
DY682 15.6 pg/ml 0.8 pg/ml 5 assays for the cost of 1
AN0110 Human HGF R&D
Systems
DY294 62.5 pg/ml 3.1 pg/ml 5 assays for the cost of 1
AN0111 Mouse IL-4 LIFE
Technologies
CMC0043 3.9 pg/ml 0.2 pg/ml 5 assays for the cost of 1
AN0112 Mouse IL-10 R&D
Systems
DY417 31.3 pg/ml 1.6 pg/ml 6 – 10
assays for the cost of 1
AN0113 Human HB-EGF R&D
Systems
DY259 15.6 pg/ml 0.8 pg/ml 2 assays for the cost of 1
AN0116 Human G-CSF R&D
Systems
DY214 31.3 pg/ml 1.6 pg/ml 5 – 10
assays for the cost of 1
AN0118 Mouse TNF-α R&D
Systems
DY410 15.6 pg/ml 0.8 pg/ml 6 – 8
assays for the cost of 1
AN0119 Human TGF-β1 R&D
Systems
DY240 31.3 pg/ml 1.6 pg/ml 40 – 80 assays for the cost of 1
AN0120 Human AKT [pS473] Invitrogen CHO0115 0.39 U/ml 0.02 U/ml 5 assays for the cost of 1
AN0121 Human IL-1α R&D
Systems
DY200 3.9 pg/ml 0.2 pg/ml 5 – 20 assays for the cost of 1
AN0122 Human IL-6 R&D
Systems
DY206 9.37 pg/ml 0.5 pg/ml ≈5 – 6.7 assays for the cost of 1
AN0123 Human TNF-α R&D
Systems
DY210 15.6 pg/ml 0.8 pg/ml 20 assays for the cost of 1
AN0124 Human IL-12 p70 R&D
Systems
DY1270 31.3 pg/ml 1.6 pg/ml 10 – 13 assays for the cost of 1
AN0126 Human IFN-γ R&D
Systems
DY285 15.62 pg/ml 0.8 pg/ml ≈ 13 assays for the cost of 1
AN0127 Human IL-13 Abcam ab47447 3.12 pg/ml 0.2 pg/ml ≈ 13 assays for the cost of 1